24.5.08

Fungal bounty: using adaptation models of fungi towards a specific antibiotic solution.

Introduction:

All forms of life on this planet are highly adaptive given a significant selective pressure and adequate resources. Symbiosis in most literature is taken as fact; the fields of environment studies and ecology deal solely with interspecies relationships that create a unique means of survival for both, each species creates a niche in an environment and fills another created by a complimenting species.

Fungi are a highly specialised kingdom. With very low food requirements, a high resilience to disease, a short reproductive cycle that is easily manipulated and a range of secondary metabolites as a means of interacting with their environment, fungi are high desirable as a niche creating group to humans.

Fungi and humans share a common pathogenic species that lives in symbiosis with us both; bacteria. Interestingly, many of the same bacteria that cause disease or death in humans do the same in fungi. This has led to a harvesting of fungi for the antibacterial secondary metabolites they produce. This is a widely accepted practice, but lacks a key strength; adaptability.

In the Ukraine bacteriophage are harvested from hospital drainage systems and stored in libraries. They are used as antibiotics in hospitals, where they actively feed on the bacteria that grows there. This premise is proved to work, though there are many reservations about its widespread use in other countries.

Given fungi's ability to reproduce quickly and spread DNA among a colony, a full colony adaptation to a pathogen can occur in as little as one generation. If this increased efficacy of pathogenic resistance could be first acquired in fungi, then passed to humans through secondary metabolite harvesting the results could be a fungal panacea; its secondary metabolites able to kill any bacteria its colony previously had contacted.

Given a bacterial library, an environment tailored for efficient fungal propagation and later stringent testing of the metabolic content of the resistant fungi produced, the results could mean a new form of antibiotic farming, where a cure is not searched for, but rather asked for with an offering of the offending pathogen.

Aim: Test the ability of fungi to adapt to various human pathogens native to Victoria.

Method:

The fungi used will vary based on adaptability of varying species.

A range of varying bacteria are harvested from culture.

Inoculations of bacterial colonies in suspension are prepared and added to the fungal food source at a stage of fungal development prior to sporing.

A subsequent test will be done on the f1 generation, with a tenfold increase in bacterial exposure. The results will be monitored closely and if pathogenesis is notably marked, a further tenfold increase will be used to inoculate the f2 generation, this will continue until there is no noted pathogenesis after exposure to the bacterium of interest.

Subsequent studies will be designed to encourage breeding between bacteria resistant fungi to optimise the spread of resistance genes.



//I'll mostly add methodology as I find what works best, and results when they become available.

Then discuss and get peer reviewed by farmer Dan.

A G Major Girl

I'm pretty comfortable, standing with my feed a shoulder's width apart, my knees slightly bent, my saxophone in my mouth and my fingers ready. I look up to the horizon, closing my eyes and starting to play.

The expression, forms ideas, words, then sound, I feel myself letting everything listen hear how I feel; what is going on in my head. I don't mind if anything is listening, I'm used to talking to myself to improve what I say to others.

I listen to what I'm saying, It's a happy and peaceful sound, one content with life, with a full belly and a clear head. Everything is soft and easy, mellow in meaning and content.

A blue chord change occurs, I'm doing a few simple scales, thinking about contact. Missing a certain feeling, unsure whether I'd ever felt it but sure I was missing it. The song gets jerky and confusing, then wildly spirals up two octaves in fifths.

I open my eyes and see a movement, I finish on a fifth, leaving the note hanging for a second then pulling my mouth off the piece and looking to see who is there.

What a wonderful person! They want me to keep playing. I'm happy to be sharing my love of music and I feel like playing something a bit happier now.

14.5.08

Koinobiont Ectoparasitoid

Une fois, une seule, aimable et douce femme,
À mon bras votre bras poli
S'appuya (sur le fond ténébreux de mon âme
Ce souvenir n'est point pâli)
- Charles Baudelaire


There is a pulse and a hunger.

I am alive, whatever that means.

The smell of the rot has become an interesting sensation.

Hunger is always present, though I don't have a stomach I am now conscious that if I had nerves connecting to it they would be telling me to feed.

I could use a steak, though at this point i'd probably eat lard. Whenever I feel a bit less hungry I guess I am feeding. It is like an orgasm. I have never been so aware of how close survival and sex are, or how nice they both feel. There are countless others here, though I can't say much to them. This unity experience is addictive; talking to only one entity doesn't cut it.

Humans are a visual species.

This taste and smell thing is somewhat new to me, I keep trying to tell my eyes to open or my body to feel. Something replies every time.

'No, not that, that isn't there.'

I'm not giving up though, my entire existence was mapped out by my eyes, losing my sight means losing a part of who I am.

'You don't need that anymore, let go, let go.'

I'm not listening.

I figure I could connect this smell to my eyes and see what is going on, it works pretty well and soon I have a rough idea of my environment. The subtle differences of smell relate to differing consciousnesses. I color code them. I have a better sense of where I end now and almost feel able to move and give myself form. When I really flex against my limits there is a sense of dismay felt around me. The rot is not happy with my independence. I feel myself pressed from all sides; compacted.

My mind is filled with images of desolation, despair, destruction, digestion, disharmony. Nothing new there, but the feeling is so much stronger than any I have felt before. I'm tempted to form a shell around myself to ward off this feeling. But resist the temptation, a wall is just as good at keeping things in as keeping things out. Instead I think desperately of thoughts that bring my peace. My first love, soft fluffy animals, fresh air, cool water, warm sun.

Feed! is shouted back in reply, my images are acceptable as things to consume, but that is not my meaning.

I go to my memory palace, a place of my own will. I retreat there, aware I am still being observed.

Things are as I left them. Five tribes fight endlessly over one side of a disc. The other side is my garden. I go there. Soft mossy grass grows across it, covering the wide expanse as far as I wish it to. This is my sanctuary, my deepest, most safe place.

'Yes, good!' comes a voice.

'Grow!'

'Feed!'

'Spread!'

'Survive!'

I am at odds with this voice. It isn't covering every part of being alive. I think of diversity, of chaos, of the difference that makes life both possible and worth living.

I feel the rot recoil in horror. It is considering my commentary. I push and feel litter resistance. Around me the rot falls away. Its smells are a bouquet of difference, I discern more colors than I have ever tasted before. All moving independently with no purpose but to live. The hunger and growth cycle is over. The rot will survive, but not as the rot.

The rot has consumed all things, all things are in the rot.

The rot is dead, long live the rot.

Phagotypical behavior

Then am I
A happy fly,
If I live,
Or if I die.
- William Blake


You're absolutely right officer, I was irresponsible and down-right immoral to disobey the order to evacuate. After all, the law exists to protect the interests of all that follow it, breaking it is an act of sedition or even treason.

The pig looks uncomfortable with my oratory, he has another five hundred or so houses to check in town before he can evac himself.

The unknown scares authority more. I remind myself this is a man in a uniform, excited by the ideals of brotherhood and protecting the state. I don't hate him for being a tool of repression of my freedom, but I certainly don't respect him for it.

I'll be on the next bus out, don't worry about it. Except I won't. I don't want him to worry about me. He wouldn't if it wasn't something he was paid to do.

The firetruck is the last thing out, cruising out at dusk, sirens blaring. Giving the town a last fanfare. I sure hope I don't light too many fires while they are gone. I grin, walking across the street in the lengthening shadows to the library to return a book.

The people left, abandoning everything they couldn't carry with them. Odd I thought, that I, the one with nothing here would stay.

The town seemed drier and older the morning after the people left. although their lives had somehow animated the wood and stone.

The next morning the first train arrived. Nobody was around to open the gate to the sheds at the end of the line. I watched from a rooftop that day as train after train crashed into the bollards.

There were no drivers, most were old freight trains, I wondered what the cargo was, making a note to check later that day.

The local store yielded a carbonated soda that promised I could win instantly by buying a bottle. I decided just to steal it instead since the novelty of money and working a cash register had worn off many years before. I chuckled as I looked under the cap. I was a winner! now to send my details somewhere and in six to eight weeks a hat with a logo on it would be mine.

The train station was a mess, derailed trains lying on their sides, some with their engines still running. It smelled pretty bad, I guessed the stench was coming from the trains. I tossed my drink bottle in a bin, wondered if anyone would ever clear it out, shrugged and wandered on, searching for an open carriage. There were biohazard signs on the closed ones and given the smell, I didn't want to find out what was in them that strongly.

I heard a rumbling that sounded like a 24 carriage train approaching at full speed.

I moved away from the tracks. A few minutes later the train came crashing into another bollard and derailed, its engine on its side, wheels still spinning. The contents of its open carriage spilled off. The smell was rancid, but the sight was worse. Hundreds of dead bodies lying in the dust before me. I spotted some humans, the clothing gave them away.

Past a certain point of decomposition all meat looks the same.

Looks like I was wrong, the train only had 23 carriages. Silly me, I giggled at the thought of guessing the number of carriages by listening to the sound a train made and wandered away.

If every train was full of rot, I thought while walking to the nursery, a forest would have all the food it needed. It rains pretty regularly here and never gets too cold. Trees were nice, but thinking about it I couldn't really get inspired by chloroplasts.

With time they'll seed themselves anyway.

I have no place helping life.

Independence of an organism is central to its adaptability and survival.

I wonder what independent organism broke the symbiotic cease fire and killed so many meatlings. The sprinkler system came on, watering the ferns I was watching. I moved out into the sun to dry myself.

I like cacti, they have a certain resolute attitude in the face of diversity, opting out of plenty to find their own niche.

My cactus is growing happily on the roof, well, sitting there. It must be saving its energy for later. But it certainly seems happy. I burnt down the church last night. Nothing against religion, just hated to see it go unused. Pity we don't have a mosque or synagogue here, I don't want to discriminate. Plus it was fun to watch a symbol of childhood repression go up in flames.

The hardware store has paint, this town is going to become pretty.

One building side in, I get tired of the old up and down motion and start throwing paint to speed things up. It gives things a much more chaotic feel.

I like that.

I went back to the nursery. Everything was dead. The water was helping wash away what was left of the ferns. I took another cacti, but I didn't get the feeling it was happy so I dropped it.

A building fell down today. I heard the sound, not sure why it did but i'm glad I wasn't in it. Might sleep outside all the same. The ground is looking more and more nasty. Can't say I would dig sleeping on it.

Asphalt and concrete, full of delicious sugar, if you look on the right day at a fresh laid piece you'll see hundreds of butterflies sitting on it feeding. I wonder if they ever get stuck, kinda like in amber.

The town smells awful this morning, more buildings fell down during the night. I slept through it apparently. Food has lost its appeal to me. This stink is enough to drive someone mad. But who? I giggle, I love me; I really crack me up sometimes.

One more day, I choke down a grain and honey snack with water. One more day without fresh air and i'm out of here. People at least don't smell this way.

I had some awful dreams last night, death, decay, a hideous pulse of hunger. I haven't been eating well recently. I pack some snack food and water into a bag and think about the best way out. The smell makes it hard. It is cloying and surrounding, breathing is difficult. I grab a toothbrush and paste off a shelf and brush my teeth, then throw them in the bag. Minty freshness doesn't seem as pleasant with this stench in contrast.

The road is cracked and some odd black goo is bubbling up through it. I start walking, scraping it off my shoes on the kerb.

Then I think, this isn't right, I can't just walk away. I crack a huge smile and break into a run.

By the time I hit the edge of town I've vomited a few times. Highly enjoyable. A building collapsed behind me, sending out a plume of dust and spurring me on.

The black stuff is everywhere in pools out here and smells like rot. When I have to cross it, it is like walking through a bog. The rot doesn't feel like there is any dirt in it though. It all seems alive. It is weird to stand knee deep in an organism.

I start wading quickly through it and get to solid ground. As I catch my breath I hear a rumble and the solid ground starts to become less so.

I am running wildly now, the ground behind me falling away and being swamped by the rot at a disgusting rate. The only thing going through my head is how much I want to be alive.

I reach the top of a rocky outcrop and look out onto a landscape of decay, the whole world is covered in this goo.

I'm atop an ancient rock, a high point solely because there is nothing up here worth eating.

Until now.

Disobeying what I know about fluid dynamics the rot starts flowing up the rocks.

The rot spreads fast, covering the rocks around me. I feel myself drawn in; the rot is accepting of all things. As I decompose I realize this is what I have always wanted. The buzzing of flies and the ancient smell of decaying cell walls, the taste of black soup made from countless billions of dead organisms feeding on each other.

This was the womb I was never aborted from.